基于线粒体12S基因全序列分析我国绵羊痒螨(兔亚种)的遗传多样性

doi:10.11843/j.issn.0366-6964.2018.11.019

Abstract

Abstract:

To explore the genetic diversity and genetic structure of Psoroptes ovis var. cuniculi, the complete sequence of mitochondrial 12S gene of P. ovis var. cuniculi isolates from 5 geographical regions (Huabei, Huadong, Huazhong, Xibei, and Xinan) in China, were obtained by PCR sequencing techniques, and evaluated the genetic diversity of P. ovis population. Eighty-nine sequences of mitochondrial 12S were obtained. The multiple alignments of the full 12S gene from 89 isolates were 657 bp. These sequences contained 76 variable sites and were classified into 50 haplotypes (H1-H50). The values of haplotypes diversitiy (Hd) and nucleotide diversity (π) were 0.931 05 and 0.005 59 respectively. There were low level of genetic differentiation, but high level of gene flow (F_st=0.042 322, Nm=5.657 372) among the 5 geographical population. In P. ovis population, the average values of Tajima's D and Fu's Fs were negative (Tajima's D=-0.928 31, Fu's Fs=-7.066 71) and there was no significant difference (P<0.05). NJ phylogenetic tree and haplotype network were both revealed that 50 haplotypes from 5 different geographical regions dispersed across different clades without associating with the geographical locations. The results revealed that there was a high level of genetic diversity, little population genetic differentiation and high gene flow in the P. ovis var. cuniculi population of China, but population structure has not formed according to rabbits breed, temperature zones and geography origin.

CLC Number:

S852.746

LI Yan-fang, GU Jiang, YUAN Yuan, XIE Yue, LAI Wei-min, YANG Guang-you, GU Xiao-bin. Genetic Diversity of Psoroptes ovis var. cuniculi Determined by the Full-length of Mitochondrial 12S Gene in China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2468-2476.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.11.019

https://www.xmsyxb.com/EN/Y2018/V49/I11/2468

References

[1] 洪霞芳. 中国兔产业发展研究[D]. 北京:北京林业大学, 2011.
HONG X F. Study on the development of rabbit industry in China[D]. Beijing:Beijing Forestry University, 2011. (in Chinese)
[2] 谢晓红,郭志强,秦应和. 我国肉兔产业现状及发展趋势[J]. 中国畜牧杂志, 2011, 47(4):34-38.
XIE X H, GUO Z Q, QIN Y H. The situation and development trend on meat-rabbit industry in China[J]. Chinese Journal of Animal Science, 2011, 47(4):34-38. (in Chinese)
[3] 赵恒章,王天有,刘保国. 肉兔耳痒螨病的诊治[J]. 中国养兔,2005(3):4-5.
ZHAO H Z, WANG T Y, LIU B G. The diagnosis and treatment on Otodectes mange of meat-rabbit[J]. Chinese Journal of Rabbit Farming, 2005(3):4-5. (in Chinese)
[4] RAFFERTY D E, GRAY J S. The feeding behaviour of Psoroptes spp. mites on rabbits and sheep[J]. J Parasitol, 1987, 73(5):901-906.
[5] NAVAJAS M, FENTON B. The application of molecular markers in the study of diversity in acarology:A review[J]. Exp Appl Acarol, 2000, 24(10-11):751-774.
[6] LAGRUE C, JOANNES A, POULIN R, et al. Genetic structure and host-parasite co-divergence:Evidence for trait-specific local adaptation[J]. Biol J Linn Soc, 2016, 118(2):344-358.
[7] SOLÓRZANO-GARCÍA B, GASCA-PINEDA J, POULIN R, et al. Lack of genetic structure in pinworm populations from New World primates in forest fragments[J]. Int J Parasitol, 2017, 47(14):941-950.
[8] 任旭琴. 遗传多样性及其研究方法[J]. 淮阴工学学报, 2002, 11(5):6-8.
REN X Q. Genetic diversity and the methods[J]. Journal of Huaiyin Institute of Technology, 2002, 11(5):6-8. (in Chinese)
[9] ZAHLER M, ESSIG A, GOTHE R, et al. Genetic evidence suggests that Psoroptes isolates of different phenotypes, hosts and geographic origins are conspecific[J]. Int J Parasitol, 1998, 28(11):1713-1719.
[10] WOFFELMAN C. DNAMAN for windows, version 5. 2. 10[R]. Netherlands:Leiden University, 2004.
[11] BURLAND T G. DNASTAR's lasergene sequence analysis software[M]//MISENER S, KRAWETZ S A. Bioinformatics Methods and Protocols. Totowa, NJ:Humana Press, 2000:71-91.
[12] TAMURA K, PETERSON D, PETERSON N, et al. MEGA5:Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Mol Biol Evol, 2011, 28(10):2731-2739.
[13] LIBRADO P, ROZAS J. DnaSP v5:A software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics, 2009, 25(11):1451-1452.
[14] EXCOFFIER L, LISCHER H E L. Arlequin suite ver 3. 5:A new series of programs to perform population genetics analyses under Linux and Windows[J]. Mol Ecol Resour, 2010, 10(3):564-567.
[15] ANDRIANTSOANIRINA V, ARIEY F, IZRI A, et al. Sarcoptes scabiei mites in humans are distributed into three genetically distinct clades[J]. Clin Microbiol Infect, 2015, 21(12):1107-1114.
[16] BARRETT L G, THRALL P H, BURDON J J, et al. Life history determines genetic structure and evolutionary potential of host-parasite interactions[J]. Trends Ecol Evol, 2008, 23(12):678-685.
[17] 黄丽琴,郭宪国. 寄生虫与宿主的协同进化关系[J]. 国际医学寄生虫病杂志, 2009, 36(1):49-54.
HUANG L Q, GUO X G. Coevolution between parasites and their hosts[J]. International Journal of Medical Parasitic Diseases, 2009, 36(1):49-54. (in Chinese)
[18] 赵习彬,王保健,简克灵,等. 基于线粒体细胞色素氧化酶b(cytb)全基因序列分析我国部分地区兔痒螨的遗传变异特征[J]. 畜牧兽医学报, 2017, 48(4):714-721.
ZHAO X B, WANG B J, JIAN K L, et al. Analysis of the characteristics of genetic variability within Psoroptes cuniculi isolates in some regions of China, inferred by mitochondrial cytochrome oxidase b gene[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(4):714-721. (in Chinese)
[19] 宋洁,张小芳,刁雪淘,等. 线粒体DNA12SrRNA、16SrRNA研究进展[J]. 安徽农学通报, 2008, 14(9):42-43, 37.
SONG J, ZHANG X F, DIAO X T, et al. Research progress of mitochondrial DNA、12SrRNA, 16SrRNA[J]. Auhui Agricultural Science Bulletin, 2008, 14(9):42-43, 37. (in Chinese)
[20] KRAKOWETZ C N, SPROAT A, LINDSAY L R, et al. Sequence variability in the mitochondrial 12S rRNA and tRNA^Val genes of Ixodes scapularis (Acari:Ixodidae) individuals shown previously to be genetically invariant[J]. Mol Cell Probes, 2015, 29(3):177-181.
[21] SKERRATT L, CAMPBELL N, MURRELL A, et al. The mitochondrial 12S gene is a suitable marker of populations of Sarcoptes scabiei from wombats, dogs and humans in Australia[J]. Parasitol Res, 2002, 88(4):376-379.
[22] GU X B, LIU G H, SONG H Q, et al. The complete mitochondrial genome of the scab mite Psoroptes cuniculi (Arthropoda:Arachnida) provides insights into Acari phylogeny[J]. Parasit Vectors, 2014, 7:340.
[23] 郝荣超,刘芳,王国华,等. 不同兔种遗传多样性与聚类分析[J]. 基因组学与应用生物学, 2015, 34(4):706-711.
HAO R C, LIU F, WANG G H, et al. Genetic polymorphism and cluster analysis in different breeds of Lepus[J]. Genomics and Applied Biology, 2015, 34(4):706-711.
[24] 季维智,宿兵. 遗传多样性研究的原理与方法[M]. 杭州:浙江科学技术出版社, 1999.
JI W Z, SU B. Principles and methodologies of genetic diversity studies[M]. Hangzhou:Zhejiang Science and Technology Press, 1999. (in Chinese)
[25] ROUSSET F. Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance[J]. Genetics, 1997, 145(4):1219-1228.
[26] 谢跃,周璇,孙韵,等. 基于线粒体12S对中国两大山系大熊猫西氏贝蛔虫种群遗传多样性的分析[J]. 兽类学报, 2015, 35(3):328-335.
XIE Y, ZHOU X, SUN Y, et al. Mitochondrial 12S-based analysis of genetic diversity of Baylisascaris schroederi in giant pandas from two mountain ranges in China[J]. Acta Theriologica Sinica, 2015, 35(3):328-335. (in Chinese)
[27] AVISE J C. The history and purview of phylogeography:A personal reflection[J]. Mol Ecol, 1998, 7(4):371-379.
[28] WRIGHT S. Evolution and the genetics of populations:Volume 4 variability within and among natural population[M]. Chicago, IL, USA:University of Chicago Press, 1978:4.
[29] PALUMBI S R. Population genetics, demographic connectivity, and the design of marine reserves[J]. Ecol Appl, 2003, 13(1):S146-S158.
[30] KOCHZIUS M, BLOHM D. Genetic population structure of the lionfish Pterois miles (Scorpaenidae, Pteroinae) in the Gulf of Aqaba and northern Red Sea[J]. Gene, 2005, 347(2):295-301.

Genetic Diversity of Psoroptes ovis var. cuniculi Determined by the Full-length of Mitochondrial 12S Gene in China

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments

[1]	CHENG Tianyin, WU Congying, LIU Yuke, DUAN Deyong. Studies on the Role of HSP70-b2 and Its 14-Mer-like Peptide from Haemaphysalis flava [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2570-2580.
[2]	HUANG Cuirui, HAO Guiying, ZHOU Yu, TIAN Yan, YANG Fusheng, TANG Li, YANG Guangyou, XIE Yue, HE Ran, XU Jing, GU Xiaobin. Recombinant Arginine Kinase of Psoroptes ovis Induces Cutaneous Eosinophil Recruitment in Rabbit [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2186-2194.
[3]	LUO Ying, PENG Meiqi, XIAO Zhengpan, LUO Chenghui, WANG Dayong, PEI Yechun. Expression of Blomia tropicalis Major Allergens Blo t 5 and Blo t 21 Induced the Production of IgG and Regulatory T Cells in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1220-1230.
[4]	GE You, ZHENG Jing, PU Shanqiu, ZHOU Yu, HUANG Cuirui, YANG Guangyou, XIE Yue, HE Ran, XU Jing, GU Xiaobin. The Transcription Level of MMP2 in Psoroptes ovis var. cuniculi and Establishment of the Indirect ELISA Based on Its Recombinant Protein [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 567-575.
[5]	QI Xiaoxiao, LI Huan, WANG Bohan, MENG Jiali, SUN Weiwei, PAN Baoliang. Research Progress on Semiochemicals of Mites and Its Application Status on Control of Dermanyssus gallinae [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3006-3013.
[6]	LIU Jinbao, CHENG Tianyin. Preliminary Identification and Functional Classification of Haemaphysalis hystricis Egg Protein Components [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2895-2904.
[7]	ZHENG Youle, CHEN Yuhang, XIE Yue, YANG Guangyou, HE Ran, GU Xiaobin. The Effects of Recombinant Macrophage Migration Inhibitory Factor of Psoroptes ovis on the Characteristic Molecules of Th1/Th2 and Th17/Treg Type Immune Responses in Rabbits Peripheral Blood Mononuclear Cells in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 594-601.
[8]	DUAN Deyong, ZHOU Hongming, CHENG Tianyin. Effects of Blood-sucking Behavior on the Microbial Community in the Midgut of Dermacentor nuttalli and Dermacentor silvarum [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 128-136.
[9]	XU Lü,CHENG Tianyin. Proteomics Analysis of Haemaphysalis flava Eggs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 627-636.
[10]	GU Jiang, LI Yan-fang, ZHAO Xi-bin, XIE Yue, LAI Wei-min, YANG Guang-you, GU Xiao-bin. Genetic Diversity of Psoroptes ovis var. cuniculi in China, Basing on the Complete Sequence of Mitochondrial 16S rRNA [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1012-1019.
[11]	WU Hui, HU Er-cha, ZHU Yu-tao, XU Zheng-mao, BAYIN Chahan. Observation on the Ultramorphology of the Various Instar Dermacentor niveus (Acari: Ixodidae) in Xinjiang, Northwestern China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1099-1109.
[12]	ZHAO Xi-bin, WANG Bao-jian, JIAN Ke-ling, YANG Guang-you, LAI Wei-min, GU Xiao-bin. Analysis of the Characteristics of Genetic Variability within Psoroptes cuniculi Isolates in some Regions of China, Inferred by Mitochondrial Cytochrome Oxidase b Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 714-721.
[13]	DUAN De-yong，CHENG Tian-yin. Bacterial Flora Analysis of Saliva and Midgut Contents from Partially or Fully Engorged Female Adult Rhipicephalus microplus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 530-537.
[14]	HU Yan-hong, YU Ling-ying, HUANG Xiao-hong. Analysis on a Transglutaminase from Haemaphysalis longicornis Expressed in Pichia pastoris [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 331-339.